INVESTIGADORES
VOLENTINI Sabrina Ines
artículos
Título:
Antifungal activity of β-carbolines on Penicillium digitatum and Botrytis cinerea
Autor/es:
OLMEDO MARÍA GABRIELA; CERIONI, LUCIANA; GONZALES, MARIA MICAELA; CABRERIZO, FRANCO MARTIN; RAPISARDA, VIVIANA A.; VOLENTINI, SABRINA I.
Revista:
FOOD MICROBIOLOGY
Editorial:
ACADEMIC PRESS LTD-ELSEVIER SCIENCE LTD
Referencias:
Lugar: Amsterdam; Año: 2017 vol. 62 p. 9 - 14
ISSN:
0740-0020
Resumen:
b-carbolines (bCs) are alkaloids widely distributed in nature that have demonstrated antimicrobial properties. Here, we tested in vitro six bCs against Penicillium digitatum and Botrytis cinerea, causal agents of postharvest diseases on fruit and vegetables. Full aromatic bCs (harmine, harmol, norharmane and harmane) exhibited a marked inhibitory effect on conidia germination at concentrations between 0.5 and 1 mM, while dihydro-bCs (harmalina and harmalol) only caused germination delay. Harmol showed the highest inhibitory effect on both fungal pathogens. After 24 h of exposure to 1 mM harmol, conidia revealed a severe cellular damage, exhibiting disorganized cytoplasm and thickened cell wall. Harmol antimicrobial effect was fungicidal on B. cinerea, while it was fungistatic on P. digitatum. Conidia membrane permeabilization was detected in treatments with harmol at sub-inhibitory and inhibitory concentrations, for both pathogens. In addition, residual infectivity of P. digitatum on lemons andB. cinerea on blueberries was significantly reduced after exposure to this alkaloid. It also inhibited mycelial growth, preventing sporulation at the highest concentration tested. These results indicate that harmol might be a promising candidate as a new antifungal molecule to control causal agents of fruit diseases.