The non-dysplastic temporal pole in patients with drug resistant temporal lobe epilepsy and hippocampal sclerosis: neuropathology and clinical aspects.

MICAELA A SANZO, HECTOR KONOPKA, ERNESTO GONZÁLEZ STIVALA, PABLO VAZQUEZ, CLAUDIO NUÑEZ, PABLO SEOANE, EDUARDO SEOANE, BRENDA GIAGANTE, SILVIA ODDO, PINCICH JP, SILVIA KOCHEN, FABIÁN C LOIDL, LUCIANA D`ALESSIO. ; MICAELA A SANZO, ERNESTO GONZÁLEZ, FABIÁN C LOIDL, LUCIANA D`ALESSIO,PABLO VAZQUEZ STIVALA

Bangkok

Congreso; 33° International Epilepsy Congress; 2019

ILAE

IntroductionThe temporal pole plays an important role in the genesis and propagation of seizures in temporal lobe epilepsy (TLE) however the mechanisms involved are not fully understood.The aim of this study was to determine the expression pattern and the clinical correlations of histopathological markers (calbindin inmunoreactivity, GFAP and NeuN) in the non-dysplasic temporal polar cortex resected durign surgical procedure of patients with temporal lobe epilepsy (TLE) and hippocampal sclerosis (HS). Methods:In all patients, clinical, neurological, EEG, video-EEG, MRI, psychiatric and neuropsychological evaluation have been performed prior to surgery. Patients with cortical dysplastic alterations detected by MRI and / or by histopathology (Blümke et al. criteria) were excluded. Follow up was determined after 2 years. Microtome histological sections form the polar temporal cortex were processed by immunohistochemistry (immunoperoxidase) with anti-NeuN, anti-calbindin antibodies (CB) and anti-acid gliofibrillary protein (GFAP). A semiquantitative analysis of the immunoreactivity was carried out using the program (Image J). The Student´s Test/Chi Square Test and Pearson correlatiosn were applied.Results: Twenty seven patients (14 women and 13 men, age 32.04 ± 9.53 years) and 5 post-mortem controls were included. The mean time of epilepsy duration was 21,85 ±10.6 years, the mean age of epilepsy onset was 9.63 ±7.9 years (16 patients had right HS (59.25%) and 11 patients had left HS (40.74%). A significant increase in the expression of GFAP and in CB were found(p