EFFECTS OF BODY CONDITION SCORE IN LIVER PROINFLAMMATORY STATE AND INSULINSIGNALING OF DAIRY CATTLE DURING THE TRANSITION PERIOD

ANGELI, E.; BARCAROLO, D; NOTARO, U.; MATILLER V.; REY F.; ORTEGA H; HEIN G.

Mar del Plata

Congreso; Reunión anual de la Sociedad Argentina de Fisiología. Sociedad Argentina de Virología (SAV). Reunión Asociación argentina de Nanomedicinas (Nanomed-ar).; 2019

SAIC

Abstract/Resumen: The transition period (last 3 weeks ofprepartum until 3 weeks postpartum) is the most critical stage inthe lactation of a dairy cow, characterized by lipid mobilization,proinflammatory state and insulin resistance during peripartum.The aim of this study was to evaluate the insulin-signalingpathway and certain proinflammatory factors in the liver andmetabolic biomarkers in plasma during the transition period incows with different body condition score (BCS). Sixteen grazingdairy cows from a commercial dairy farm were classifiedaccording to BCS using the 5-point scale as high BCS (HBCS,>3.5, n = 8) and low BCS (LBCS, < 3.5, n = 8). Blood and liverbiopsies were sampled at -14, 4, 14 and 28 days relative toparturition. The concentrations of non-esterified fatty acids(NEFA), beta-hydroxybutyrate (BHB), glucose, triglycerides,insulin and liver triglycerides content werespectrophotometrically measured. Also, liver protein expressionof relevant insulin signaling components: insulin receptor (IR),insulin receptor substrate 1 (IRS-1), total and phosphorylatedprotein kinase B (Akt and p-Akt); along with tumor necrosisfactor alpha (TNF-alpha), interleukin-6 (IL-6), and nuclear factorkB(NF-kB) were measured by Western blot. The obtained resultsshown that cows with HBCS had greater BHB concentration thanLBCS (p