INVESTIGADORES
MATILLER Valentina
congresos y reuniones científicas
Título:
Oxidative stress biomarkers and pathways of hepatic lipid metabolism in dairy cattle supplemented with minerals and vitamins during the transition period
Autor/es:
ANGELI, E.; BARCAROLO, D; CANAVESIO, J; REY, F; MATILLER V.; ORTEGA, H; HEIN, G.
Lugar:
Mar del Plata
Reunión:
Congreso; Reunión anual de Sociedades de Biociencias. LXVII Reunión Anual de SAIC.; 2022
Institución organizadora:
Sociedad Argentina de Investigación Clínica
Resumen:
The transition period is the most critical stage in the lactation of a dairy cow, characterized by lipid mobilization and a great metabolic demand that could increase oxidative stress (OS). Therefore, this study aimed to evaluate some OS parameters and pathways of hepatic lipid metabolism in cows supplemented with vitamins and minerals during this period. The supplemented group (SG; n = 11) received a subcutaneous dose of 5 ml of the vitamin supplement ADAPTADOR® Vit and 5 ml of the mineral supplement ADAPTADOR® Min (Biogenesis Bagó, Bs. As.; vitamin A palmitate 3.5% and vitamin E acetate 5%, copper edetate 1%, zinc edetate 4%, manganese edetate 1% and sodium selenite 0.5%) on -60, -30 and 7 days relative to calving. The control group (CG; n = 11) received two injections of 5 ml of 0.9% sodium chloride solution. Blood, urine and liver biopsies were sampled at -21, 7 and 21 days relative to calving, and malondialdehyde (MDA) was evaluated by mass spectrometry as an OS biomarker. In addition, serum concentrations of non-esterified fatty acids (NEFA), beta-hydroxybutyrate acid (BHBA) and the total antioxidant status (TAS) were determined by using commercial colorimetric assay kits. Finally, triacylglycerol content was measured spectrophotometrically, and protein expressions of carnitine palmitoyltransferase-1 (CPT1), acyl-CoA oxidase-1 (ACOX1); diacylglycerol acyltransferase-1 (DGAT1) and nuclear receptor PPAR-alpha were evaluated in liver biopsies by Western blot. No differences in NEFA and BHBA concentrations were observed (p > 0.05). However, a lesser hepatic triacylglycerol content was registered in cows of the SG (p < 0.05). Regarding liver enzymes, a greater protein expression of CPT-1 was observed in the SG (p < 0.05) without differences in the other enzymes evaluated (p > 0.05). Similar MDA concentrations and TAS were observed (p > 0.05). These results suggest that mineral and vitamin supplementation could influence liver physiology by improving the adaptation of the dairy cow during the transition.