INVESTIGADORES
SZPILBARG Natalia
congresos y reuniones científicas
Título:
AQUAPORIN-9 EXPRESSION IN THE MITOCHONDRIA OF PREECLAMPTIC PLACENTAS
Autor/es:
FERNÁNDEZ, NAZARENA; SIERRA MATÍAS N; SZPILBARG, NATALIA; FAVALE, NICOLÁS; MARTÍNEZ, NORA; DAMIANO, ALICIA ERMELINDA
Lugar:
Buenos Aires
Reunión:
Congreso; Congreso Anual SAFIS 2023; 2023
Institución organizadora:
Sociedad Argentina de Fisiología
Resumen:
Introduction: Preeclampsia is a gestational hypertensive disorder where increased oxidative stress leads to mitochondrial dysfunction. During the syncialization, trophoblast mitochondria undergo morphological and functional changes resulting in two mitochondria subpopulations the "heavy/large" fraction and the "light/small" one. Previously, we described an increase in AQP9expression in the trophoblast membranes of preeclamptic placentas. However, its function is unknown. Emerging evidence shows that AQP9 may act as peroxyporin. Recently, we found that AQP9 also localizes in the trophoblast mitochondria of normal placentas with a high expression in the “heavy/large” mitochondrial fraction whereas it was almost undetectable in the"light/small" one. Here, we aimed to study the localization of AQP9 in the trophoblasts from preeclamptic placenta.Materials and methods: Placentas were obtained from healthy-term (n=3) and preeclamptic (n=3) pregnancies. Microsomal and the two mitochondrial fractions were isolated from placental tissue by differential centrifugation. The presence of functional mitochondria was evaluated by MitoTracker. AQP9 expression was analyzed by Western blot. Co-localization of AQP9 andcytochrome-C was assessed by double-immunofluorescence and confocal microscopy. Results: AQP9 expression was significantly increased in the microsomal and in heavy/large mitochondrial fraction (p