INVESTIGADORES
SZPILBARG Natalia
congresos y reuniones científicas
Título:
EVIDENCE FOR OXYGEN-MEDIATED CONTROL OF CFTR AND CAVEOLIN-1 IN HUMAN PLACENTAS
Autor/es:
SZPILBARG NATALIA; SEHAYIAN ABRIL; MEDINA YOLLYSETH; DI PAOLA MAURICIO; FARINA MARIANA; MARTÍNEZ NORA; DAMIANO, ALICIA E.
Lugar:
Buenos Aires
Reunión:
Congreso; Reunión Conjunta de Sociedades de Biociencias; 2017
Institución organizadora:
Reunión Conjunta de Sociedades de Biociencias
Resumen:
It has been proposed that intermittent placental perfusion, secondaryto deficient trophoblast invasion of the endometrial arteries,leads to an ischemia-reperfusion type insult in preeclamptic placentas(PE). Such variations in oxygenation can further alter the syncytiotrophoblastfunctions. We have previously reported that thecystic fibrosis transmembrane conductance regulator (CFTR) issignificantly reduced in PE. In addition, we have recently reportedalterations in the membrane lipid composition of the apical membraneof syncytiotrophoblast (hST) of PE. Therefore, we also detectedthat the expression Caveolin-1, the major structural component ofcaveolas, was reduced in these membranes.Our aim was to identify the mechanisms implicated in the regulationof CFTR and Caveolin-1(Cav-1) expression. We hypothesizedthat intermittent hypoxia may be responsible for the altered expressionof both proteins.Localization of CFTR and Cav-1 expressions were studied bydouble immunofluorescence in normal placentas and PE (n=13). Tostudy the role of oxygen, explants from normal placenta were culturedin normoxia, hypoxia, and hypoxia/reoxygenation (H/R) andthe mRNA and protein expressions of CFTR and Caveolin-1 weredetermined.In normal placentas, both proteins co-localized in the apical membraneof the hST, but the colocalization was lost in PE. CFTR expressiondecreased 2-fold (n=6; p