INVESTIGADORES
SZPILBARG Natalia
congresos y reuniones científicas
Título:
New insights in the regulation of placental AQP9: oxygen tension modulates AQP9 expression in human placenta
Autor/es:
CASTRO-PARODI MAURICIO; SZPILBARG NATALIA; DIETRICH VALERIA; DI PAOLA MAURICIO; MASKIN BERNARDO; FARINA MARIANA; DAMIANO ALICIA ERMELINDA
Lugar:
Foz de Iguazú
Reunión:
Congreso; V SLIMP - IV LASRI Meeting 2013; 2013
Institución organizadora:
SLIMP-LASRI
Resumen:
The response of AQP9 to changes in oxygen tension in trophoblast is still unknown. Our aim was to establish whether alterations in oxygen may modulate placental AQP9. We analyzed human AQP9-gene to find putative HIF-1 binding sites. Explants from normal placentas were cultured at different concantrations of oxygen or with CoCl2. AQP9 expression and water uptake were determined. Lipid membrane composition was also studied. Although fourteen consensus HIF-1 binding sites were found in AQP9 gene, protein expression decreased abruptly when HIF1a was expressed by deprivation of oxygen of CoCl2 stabilization. Consequently, water uptake showed an important decrease. In contrast, after reoxygenation, HIF1a was undetectable while AQP9 increased significantly. Interentingly, phospholipid membrane comoposition of trophoblast was altered after treatments. Our findings suggest that oxygen may modulate placental AQP9 expression which seems to be independent of water flux.