6. Polyclonal anti-alpha3 (IV)NC1 antibodies (Ab) from goodpasture (GS) and transplanted alport (AL) patients show high affinity to the alpha3(IV)NC1 epitope.

4. RUTGERS A, MEYERS KEC, CANZIANI GA, KALLURI R, LIN J, MADAIO MP.

Denver, Colorado, USA

Congreso; Annual Meeting of the American Society of Nephrology; 1998

ASN

Anti-glomerular basement membrane (anti- glomerularAb deposition has the potential to produce continuous inflammation, progressive glomerulonephritis (RPGN) that often results in irreversible loss of renal function and renal failure. We hypothesized one of the incumbent factors could be the persistence of the autoantibody-a3(IV) collagen interactions that lead to antibody deposition, thereby perpetuating inflammation. To address this hypothesis, the binding kinetics of human anti-GBM antibodies (Ab) to a3(IV)NC1 were evaluated using an optical biosensor interaction analysis. We measured the dissociation rates of these serum antibodies bound to a3(IV)NC1 collagen but not to the control a1(IV)NC1. Purified human IgG did not bind to either of these antigens. The data supports the continuous inflammation and fulminant nature of this disease as well as its resistance to immunosupression and removal of circulating antibodies.