Development of Attenuated Viral Strains

BERENSTEIN M, CARLUCCI MJ

Congreso; Bio International Convention; 2012

Technology description: The present technology consists of a novel methodology for producing attenuated viral strains, which comprises contacting at least one sulphated polymer and a virus susceptible to the inhibition of said polymer, via about fifteen o more successive passages of the virus with increasing polymeric concentrations. Said amenable virus is characterized by the method of reducing viral plaques and the strain resulting from the attenuated virus has stable phenotypic and genotypic characteristics, different from that of the wild type strain. Sulphated polymers inhibit a wide range of enveloped and naked viruses, generally, by chemically blending with heparin sulphate, competing with it. Heparin sulphate binds the virion and is responsible of the initial binding between wild type virus and host cells.