INVESTIGADORES
REY Osvaldo
artículos
Título:
Protein kinase D3 activation and phosphorylation by signaling through G alpha q.
Autor/es:
YUAN J, REY O, ROZENGURT E.
Revista:
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
Editorial:
ACADEMIC PRESS INC ELSEVIER SCIENCE
Referencias:
Año: 2005 p. 270 - 276
ISSN:
0006-291X
Resumen:
PKD is the founding member of a novel protein kinase family that also
includes PKD2 and PKD3. PKD has been the focus of most studies up to
date, but little is known about the mechanisms that mediate PKD3
activation. Here, we demonstrate that PKD3 immunoprecipitated from
COS-7 cells transfected with a constitutively active G alpha q subunit
(alpha(q)Q209L) exhibited a marked increase in basal activity. Addition
of aluminum fluoride to cells co-transfected with PKD3 and wild type G
alpha(q) also induced PKD3 activation. G alpha(q)-mediated PKD3
activation is associated with persistent translocation of PKD3 from
both cytosol and nucleus to plasma membrane. Expression of a
COOH-terminal fragment of G alpha q that acts in a dominant-negative
fashion attenuated PKD3 activation in response to bombesin receptor
stimulation. Our results indicate that G alpha q activation is
sufficient to stimulate sustained PKD3 activation and show that the
endogenous G alpha q is a major component in the signaling pathway that
mediates bombesin-induced PKD3 activation.