INVESTIGADORES
REY Osvaldo
artículos
Título:
Intracellular redistribution of protein kinase D2 in response to G-protein-coupled receptor agonists.
Autor/es:
REY O, YUAN J, ROZENGURT E.
Revista:
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
Editorial:
ACADEMIC PRESS INC ELSEVIER SCIENCE
Referencias:
Año: 2003 p. 817 - 824
ISSN:
0006-291X
Resumen:
The protein kinase D (PKD) family consists of three serine/threonine
protein kinases: PKC mu/PKD, PKD2, and PKC nu/PKD3. While PKD has been
the focus of most studies to date, no information is available on the
intracellular distribution of PKD2. Consequently, we examined the
mechanism that regulates its intracellular distribution in human
pancreatic carcinoma Panc-1 cells. Analysis of the intracellular
steady-state distribution of fluorescent-tagged PKD2 in unstimulated
cells indicated that this kinase is predominantly cytoplasmic. Cell
stimulation with the G protein-coupled receptor agonist neurotensin
induced a rapid and reversible plasma membrane translocation of PKD2 by
a mechanism that requires PKC activity. In contrast to the other PKD
isoenzymes, PKD2 activation did not induce its redistribution from the
cytoplasm to the nucleus. Thus, this study demonstrates that the
regulation of the distribution of PKD2 is distinct from other PKD
isoenzymes, and suggests that the differential spatio-temporal
localization of these signaling molecules regulates their specific
signaling properties.