INVESTIGADORES
REY Osvaldo
artículos
Título:
Nuclear retention of M1 protein in a temperature-sensitive mutant of influenza (A/WSN/33) virus does not affect nuclear export of viral ribonucleoproteins.
Autor/es:
REY O, NAYAK DP.
Revista:
JOURNAL OF VIROLOGY
Editorial:
AMER SOC MICROBIOLOGY
Referencias:
Año: 1992 p. 5815 - 5824
ISSN:
0022-538X
Resumen:
We investigated the properties of ts51, an influenza virus (A/WSN/33)
temperature-sensitive RNA segment 7 mutant. Nucleotide sequence
analysis revealed that ts51 possesses a single nucleotide mutation,
T-261----C, in RNA segment 7, resulting in a single amino acid change.
Phenylalanine (position 79) in the wild-type M1 protein was substituted
by serine in ts51. This mutation was phenotypically characterized by
dramatic nuclear accumulation of the M1 protein and interfered with
some steps at the late stage of virus replication, possibly affecting
the assembly and/or budding of viral particles. However, although M1
protein was retained within the nucleus, export of the newly
synthesized viral ribonucleoprotein containing the minus-strand RNA
into the cytoplasm was essentially the same at both permissive and
nonpermissive temperatures. The roles of M1 in the export of viral
ribonucleoproteins from the nucleus into the cytoplasm and in the virus
particle assembly process are discussed.