BICH Gustavo Angel
Evaluation of rice by-products, incubation time, and photoperiod for solid state mass multiplication of the biocontrol agents Beauveria bassiana and Metarhizium anisopliae
BICH G. A.; CASTRILLO M. L.; VILLALBA L. L.; ZAPATA P. D.
Faculty of Agronomy, Estonian Agricultural University
The success of biological control of insect pests depends not only on the isolation, characterization and pathogenicity, but also on the success of the massive production of the microbial agents. The biological control strategy using entomopathogenic fungi like B. bassiana and M. anisopliae can only be useful if practical and economic methods of massive multiplication are available. Rice by-products like broken rice grains, rice hulls and their combination was evaluated for solid state multiplication of B. bassiana and M. anisopliae. The influence of photoperiod and incubation time in the production of conidia was also evaluated. This study showed that, broken rice was the most productive substrate for conidial production of both fungal genera, with a yield of 4.62 x 107 and 2.22 x 106 conidia g-1 respectively. Also, under the evaluated solid state multiplication conditions, the best conidia production was achieved with a photoperiod of 24 h of light for B. bassiana (with 4.43 x 107 conidia g-1) and M. anisopliae (with 1.35 x 106 conidia g-1). Results demonstrated that the two fungal species could feasibly multiplied with high yields of conidia on agro-industrial by-products using solid state culture and regulating some culture conditions.