INVESTIGADORES
BALDO Juan Diego
artículos
Título:
Sex chromosomes in the Vizcacheras? White-lipped frog, Leptodactylus bufonius (Anura: Leptodactylidae)
Autor/es:
SCHNEIDER, ROSIO; FERRO, JUAN MARTÍN; REINKO, IVANA; BOERIS, JUAN; CARDOZO, DARIO; BALDO, DIEGO
Revista:
ANAIS DA ACADEMIA BRASILEIRA DE CIENCIAS.
Editorial:
ACAD BRASILEIRA DE CIENCIAS
Referencias:
Año: 2021 vol. 93 p. 1 - 12
ISSN:
0001-3765
Resumen:
Cytogenetic analyses were performed on specimens of Leptodactylus bufonius from different localities in Argentina. Mitotic chromosomes were studied with Giemsa and differential staining techniques (Ag-NOR, C-banding, and CMA3/DAPI) and fluorescence in situ hybridization with a 18S rDNA probe. All specimens showed karyotypes with 2n = 2x = 22 and FN = 44. Secondary constrictions were present in the long arm of chromosome pair 8, coincident with Ag-NOR and hybridization signals of 18S rDNA probe. The C-banding technique evidenced an important amount of heterochromatin with a sex-linked pericentromeric band in the short arm of chromosome pair 4. This heterochromatic band was heteromorphic in males but present in both homologues of females and was CMA3 positive (DAPI negative) at fluorescence staining. The occurrence of heteromorphic XY sex chromosomes in L. bufonius is the second known case in Leptodactylus and the fifth within the speciose family Leptodactylidae.