INVESTIGADORES
ONDARZA Paola Mariana
congresos y reuniones científicas
Título:
Total antioxidant capacity as a measurement of reactive oxygen species exposure in fish (Cyprinus carpio) from Negro river, Argentinian Patagonia.
Autor/es:
SHIMABUKURO V.M.,; ONDARZA P.M.; MITTON F.; MONSERRAT J,; GONZALEZ M.; MIGLIORANZA K.S.B.
Lugar:
Portland, USA
Reunión:
Congreso; 31th Annual Meeting of Society of Environmental Toxicology and Chemistry (SETAC) North America.; 2010
Institución organizadora:
North America Society of Environmental Toxicology and Chemistry (SETAC)
Resumen:
Negro River consititutes the most important waterbody along north of Patagonia Argentina. Agricultural and fruit production are the main activities developed in the area. Also urban settlement are common along the Valleys. Consequently a great variety of pollutants are discharged to the river, and a predominance of pesticides are found. Among them, organochlorine pesticides (OCPs), such as DDTs and endosulfan were hardly used in the region and particularly the metabolites DDE and endosulfan sulfate are the main OCPs found in all matrixes (soil, sediment, macrophytes). These compounds are persistent, lipophilic and are known to enhance Reactive Oxygen Species (ROS) formation. Determination of total antioxidant capacity (ACAP), is very important for the understanding of how antioxidants interact with ROS. The impact of these pesticide on aquatic environment is a field that deserve more attention. For this purpose, fish (Cyprinus carpio) were chosen as biomonitor of pesticide impact on the aquatic system in order to evaluate the levels of OCPs in different organs and the ACAP as a measurement of defense against ROS. Organisms were collected from the medium valley (MV) and control site (CS). Gills, liver, brain and gonads were subsampled for OCPs analyses by GC-17A Shimadzu, and for ACAP determination by fluorometric method. Results indicate that samples from MV presented the highest concentration of OCPs in all organs. Moreover differences between sexes was also observed. Liver and gonads presented the highest levels of pesticides being DDE the main compound found. When ACAP were evaluated a clear difference between MV and CS was observed, showing higher antioxidant capacity in organisms from MV. Therefore despite organisms from MV present higher OCPs levels, which would be precursor of ROS, particular mechanisms lead to these organisms to develop strategies to obtain higher capacity to face different ROS. Among organs it is important to mention that brain showed the highest antioxidant capacity and it is could be related to the importance of biochemical responses to xenobiotic bioaccumulation by this organ. Particularly, when females and males were compared, females from MV showed higher ACAP measurement in gonads and liver. However, in CS non differences were found between sexes in all organs. In conclusion, measurements of bioaccumulation and biomarker responses in fish offer great promises for providing information to environmental monitoring programs.