INVESTIGADORES
GONZALEZ Maria Elisa
congresos y reuniones científicas
Título:
Polyamine metabolism during sclerotial development of Sclerotinia sclerotiorum
Autor/es:
GÁRRIZ, A; GONZALEZ, ME; MONTES, M; MENÉNDEZ, A; PIECKENSTAIN, FL; RUIZ-HERRERA, J; RUIZ, OA
Lugar:
Iguazú, Misiones, Argentina
Reunión:
Congreso; XL Reunión anual Sociedad argentina de investigación en bioquímica y biología molecular; 2004
Institución organizadora:
Sociedad Argentina de Investigación en Bioquímica y Biología Molecular
Resumen:
Polyamines (putrescine, spermidine and spermine) are polycationic compounds found in all living organisms. They have shown to be essential for growth and morphogenesis of several fungi. We have previously studied polyamine metabolism on different steps of the life-cycle of Sclerotinia sclerotiorum (1, 2). In this work we evaluated polyamines requirement for sclerotial development, and the effect of polyamine biosynthesis inhibitors on this process. Neither á-difluoro-methylornithine nor cyclohexylamine, inhibitors of putrescine and spermidine biosynthesis respectively, reduced the number of sclerotia produced in vitro. By the contrary, mycelial growth was impaired. Furthermore, the activity of key polyamine biosynthetic enzymes, as well as free spermidine and spermine levels, diminished during sclerotial maturation. These results suggest that even though vegetative growth requires suitable levels of polyamines, sclerotial development in turn seems to be less dependent on polyamine biosynthesis. Further considerations on polyamine metabolism concerning sclerotia maturation and the potential use of polyamine biosynthesis inhibitors to control sclerotial-forming fungal phytopathogens are discussed. 1-Garriz et al. Archives of Microbiology 180: 169-175. 2-Garriz et al. New Phytologist 161: 847-854.