Polyamine catabolism and H2O2 production in plant- pathogen interactions

RODRÍGUEZ-KESSLER, M; JASSO ROBLES, FI; GONZALEZ, ME; PIECKENSTAIN, FL; RAMÍREZ-GARCÍA, JM; GUERRERO-GONZÁLEZ, ML; JIMÉNEZ-BREMONT, JF

Puerto Vallarta

Congreso; XVII National Congress on Plant Biochemistry and Molecular Biology. X Joint Mexico - U. S. Symposium; 2017

Polyamines(PAs) are low molecular weight aliphatic polycations, with important roles in plant growth, development and stress responses. Polyamine oxidases(PAO) are FAD- dependent enzymes that catabolize a variety of higher PAs such as spermidine, spermine and thermospermine. Hydrogen peroxide(H2O2) is an important product of PA catabolism, thereby linking PA degradation with cell signaling, defense responses and programmed cell death. Herein, the importance of PA catabolism regulation and the production of H2O2 in biotrophic(maize-Ustilago maydis) and hemibiotrophic(Arabidopsis-Pseudomonas) interactions are presented. In both cases, a differential expression of members of the maize and Arabidopsis PAO gene families were noticed. In maize, our data suggest that H2O2 produced through PA catabolism by ZmPAO1(encoding en extracellular enzyme) plays an important role in tumor development during the maize-U. maydis interaction. In the case of Arabidopsis, AtPAO1 and AtPAO2 genes(encoding citosolic and peroxisomal enzymes, respectively) are important in defense responses against Pseudomonas. Alteration in PA catabolism(using T-DNA insertional lines) affects ROS production and the activities of RBOH, SOD and catalase enzymes. Our data indicate that PA catabolism is modulated differentially in monocot and dicot in plant-pathogen interactions.