High throughput (RNA-Seq) screening for biomarkers of azinphos methyl (AZM) exposure in Rhinella arenarum.

MARDIROSIAN, MARIANA; CESCHIN, DANILO GUILLERMO; PIRES, NATALIA SUSANA; LASCANO, CECILIA INÉS; VENTURINO ANDRÉS

Congreso; 9th Congress of Toxicology in Developing Countries and XIX Congresso Brasileiro de Toxicología; 2015

Azinphos methyl (AZM) is the main organophosphate pesticide applied in Río Negro-Neuquén valley in Argentina. In this sense, it is necessary to control the levels of residues and environmental impact of pesticides. Traditional ecotoxicological biomarkers like reduced glutathione (GSH) levels and the activities of Glutathione S transferase (GST) and Catalase (CAT) are commonly used for biomonitoring, however they may fail for sensitivity and unspecifically respond to oxidative stress. Native species is a preferred resource for its ecological significance. In this sense, we have evaluated pesticide effects on common toad R. arenarum larvae. In this work, we screen for molecular biomarkers using RNA-Seq technology and compare the response with traditional biomarkers, in order to develop new specific and sensitive biomarkers.