INVESTIGADORES
LASCANO Cecilia Ines
congresos y reuniones científicas
Título:
Differential effects of two organophosphorus pesticides on polyamine metabolism in toad embryos.
Autor/es:
PIRES, NATALIA SUSANA; LASCANO, CECILIA; VENTURINO, ANDRÉS
Lugar:
Córdoba
Reunión:
Congreso; LII Reunión Anual Sociedad Argentina de Investigación en Bioquímica y Biología Molecular (SAIB).; 2016
Resumen:
Organophosphorus pesticides like chlorpyrifos (CPF) and azinphosmethyl (AZM) are widely applied in the fruit-producing valley of Río Negro and Neuquén. Amphibian embryos are particularly sensitive to numerous environmental contaminants. Polyamines (PA) are essential for normal cell growth and differentiation, being highly regulated through the balance in the first-step synthesizing enzyme ornithine descarboxylase (ODC) and degradation by polyamino-(APAO), diamino-(DAO) and spermine (SMO) oxidases. Our objective was to evaluate the effects of AZM and CPF on PA oxidation in R. arenarum embryo, measuring oxidase activities through kinetic spectrofluorometry analysis. SMO and DAO activities were increased by 9 mg/L AZM (62% and 69%) in early embryos (3d), while the effect was highly noticeable on APAO (4-fold increase). CPF did not affect oxidases in early stages. At the end of embryo development AZM effect was smoother on DAO (20% increase) but sustained on SMO by 2 mg/L exposure (70% increase). Only a minor decrease (10%) was caused by CPF. According with our previous results, alterations caused by AZM in late embryos may be related to an increase in putrescine driven both by ODC induction and spermidine/ spermine oxidation back to diamine. Conversely, the decrease in PA reported in early embryos exposed to CPF would be only related to the decrease in ODC as oxidases are not affected.