INVESTIGADORES
LASCANO Cecilia Ines
congresos y reuniones científicas
Título:
Effect of azinphos methyl on polyamine levels during Bufo arenarum embryonic development
Autor/es:
LASCANO CECILIA; COCHÓN ADRIANA; VERRENGIA, NOEMÍ; FERRARI ANA; VENTURINO ANDRÉS
Reunión:
Congreso; XLII Reunión Anual Sociedad Argentina de Investigación en Bioquímica y Biología Molecular (SAIB); 2006
Resumen:
The polyamines (PA) putrescine (Pu), spermidine (Sd) and spermine (Sm) are ubiquitous polycations involved in cell growth and differentiation. On the other hand, azynphos methyl (AM) is an organophosphate insecticide widely applied in the Alto Valle region. Our objective is to determine the effects of AM on PA levels during the embryogenesis of the common toad (Bufo arenarum). Embryos were exposed to 9 mg/l of AM since fertilization and until complete development (11 days), with medium replacement every 48 h. PA levels were determined by HPLC at different days of development. The three PA showed a continuous increase during development until complete operculum, being Pu the most relevant followed by Sd. Sm was detectable only in the last stages. AM induced a significant increase on Pu levels (131%) on late developmental stages, while caused a significant drop in Sd (30%) and Sm (below detection limit) levels. AM might block Sd and Sm synthesis, increase their oxidation by polyamine oxidase, or both thus increasing Pu levels and disturbing normal embryonic development. Pu might also increase as a secondary response to stress. Oxidation hypothesis is in agreement with the oxidative stress due to AM exposure during B. arenarum embryonic development suggested by the responses of superoxide dismutase, catalase, glutathione reductase and glutathione transferase activities.