ASL, a natural immobilized lipase found in Araujia sericifera latex

DI SANTO METZLER, P.; FAIT, M.E.; FORESTI, M.L.; MORCELLE, S.R.

Búzios

Simposio; VII Workshop on Biocatalysis and Biotransformations and 1er Simposio Latinoamericano de Biocatalisis y Biotransformaciones; 2014

SAByB

Lipases are ubiquitous in nature and are produced by plants, animals and microorganisms. Lipases of microbial origin (fungal and bacterial) are the most commonly used enzymes in biotechnological applications and in organic chemistry. Recently, much research has been devoted to the characterization and use of plant lipases obtained from latex, with a view to provide an alternative to the microbial lipases produced using genetic engineering methods. In the current contribution, the hydrolytic and synthetic activity of lipase from the insoluble fraction of latex obtained from fruits of Araujia sericifera (ASL) Brot. (Apocynaceae), are characterized by use of target substrates. ASL activity was compared with Novozym 435, a commercial immobilized lipase used as standard.