INVESTIGADORES
DELSOUC Maria Belen
congresos y reuniones científicas
Título:
Coeliac ganglion modulates the antisteroidogenic response of ovarian nitric oxide in the first rat proestrus
Autor/es:
DELSOUC MB; MORALES LD; CASAIS M; DELGADO SM; RASTRILLA AM
Lugar:
Mendoza
Reunión:
Congreso; XXXI Reunión Anual de la Sociedad de Biología de Cuyo; 2013
Institución organizadora:
Sociedad de Biología de Cuyo
Resumen:
The ex vivo coeliac ganglion-superior ovarian nerve-ovary (CG-SON-O) system allows to study the relationship between the peripheral nervous system and the reproductive function. It has been shown that stimulating the CG with Acetylcholine (Ach), increases the ovarian levels of the neurotransmitter nitric oxide (NO). The aim of this work was to study if inhibitors of nitric oxide synthase (NOS) and an NO donor in ovarian compartment, with and without addition of Ach 10-6 M in CG, modify the release of NO and progesterone (P4), in the first rat proestrus. The system was incubated in Krebs-Ringer buffer at 37°C. The addition of selective and non-selective inhibitors of inducible NOS, aminoguanidine 400 μM (AG) and L-nitroarginine methyl ester 100 μM (L-NAME), respectively; and NO donor, sodium nitroprusside 100 μM (SNP), were studied separately. NO (by the Griess technique) and P4 (by RIA) were determined in the ovarian compartment at 30, 120 and 180 min of incubation. One-way ANOVA and Tukey test were used. In absence of Ach, AG inhibited NO at 30´, 120´ (p