INVESTIGADORES
DELSOUC Maria Belen
congresos y reuniones científicas
Título:
Contribution of the ganglionic nitric oxide/ nitric oxide synthase system in the ovarian nitric oxide release
Autor/es:
DELSOUC MB; DELGADO SM; RASTRILLA AM
Lugar:
San Luis
Reunión:
Congreso; XXX Reunión Anual de la Sociedad de Biología de Cuyo; 2012
Institución organizadora:
Sociedad de Biología de Cuyo
Resumen:
It is known the presence of the nitric oxide/nitric oxide synthase (NO/NOS) system in coeliac ganglion. Using the ex vivo coeliac ganglion-superior ovarian nerve-ovary (CG-SON-O) system, has been shown that the stimulation of CG with Acetylcholine (Ach) increases NO release in the ovarian compartment. The aim of this work was to study, in the first rat proestrus, if the presence in CG of a NO donor and inhibitors of NOS, modifies the NO release, with and without Ach 10-6M in GC. The system was incubated in Krebs-Ringer buffer at 37°C. NO donor, sodium nitroprusside (SNP) 100 µM and inhibitors of NOS: non-selective L-nitroarginine methyl ester (L-NAME) 100 µM and the selective of iNOS (inducible), aminoguanidine (AG) 400 µM, were added in CG. Ovarian nitrites were determined by the Griess method (nmol/mg ovary) at 30, 60 and 120 min. We used one-way ANOVA and Tukey test (p