Nitric oxide and progesterone: response from ovary without neural influence

MORALES LD; DELSOUC MB; CASAIS M; DELGADO SM; RASTRILLA AM

Mendoza

Congreso; XXXI Reunión Anual de la Sociedad de Biología de Cuyo; 2013

Sociedad de Biología de Cuyo

Using the coeliac ganglion-superior ovarian nerve-ovary system (Sys), we show that nitric oxide (NO) modifies the ovarian steroidogenesis. Since the NO/nitric oxide synthase (NOS) system is present in coeliac ganglion and ovary, the aims of this work in the first rat proestrus were: 1) to study the effects of NOS inhibitors and a NO donor on the release of NO and progesterone (P4) in ovary alone (Ov) (without neural influence), 2) to compare NO and P4 responses between Ov and previous results in the Sys. The Ov was incubated in Krebs-Ringer buffer (basal condition) at 37°C. Inhibitors of NOS: aminoguanidine 400 μM (AG) and L-nitroarginine methyl ester 100 μM (L-NAME), selective and non-selective of inducible NOS, respectively; and NO donor: sodium nitroprusside 100 μM (SNP), were added in Ov. NO (by the Griess technique) and P4 (by RIA) were determined at 30´, 120´ and 180´. ANOVA and Tukey test were used. In basal conditions, Ov showed higher levels of NO (p