INVESTIGADORES
DELSOUC Maria Belen
congresos y reuniones científicas
Título:
Nitric oxide in coeliac ganglion and its effect on ovarian progesterone secretion in the first rat proestrus
Autor/es:
DELSOUC MB; MORALES LD; DE LA VEGA M; DELGADO SM; CASAIS M
Lugar:
San Luis
Reunión:
Congreso; XXXII Reunión Anual de la Sociedad de Biología de Cuyo; 2014
Institución organizadora:
Sociedad de Biología de Cuyo
Resumen:
Using the coeliac ganglion-superior ovarian nerve-ovary system (CG-SON-O), we showed that stimulating the CG with acetylcholine (Ach) decreases ovarian progesterone (P4) release. Several studies suggest that nitric oxide (NO) modulates cholinergic neurotransmission. The aim of this work was to study if inhibitors of nitric oxide synthase (NOS) and a NO donor in CG, with and without addition of Ach 10-6 M, modifies P4 release and gene expression of 3β-HSD and 20α-HSD enzymes (P4 synthesis and degradation enzymes, respectively), in ovary. The system was incubated in Krebs-Ringer buffer at 37°C. The addition of selective and non-selective inhibitors of inducible NOS, aminoguanidine 400 µM (AG) and L-nitroarginine methyl ester 100 µM (L-NAME) respectively, and NO donor, sodium nitroprusside 100 µM (SNP), were studied separately. P4 was determined by RIA in ovarian compartment at 30´, 120´ and 180´ incubation times. Ovarian gene expressions of 3β-HSD and 20α-HSD were assessed by RT-PCR at 180´. One-way ANOVA and Tukey test were used (p