Acetylcholine in coeliac ganglion increases nitric oxide release with implications on ovarian estradiol secretion

DELSOUC MB; VALLCANERAS S; DELGADO SM; CASAIS M

San Miguel de Tucumán

Congreso; 3ra Reunión Conjunta de Sociedades de Biología de la República Argentina; 2015

Using the ex vivo coeliac ganglion-superior ovarian nerve-ovary (CG-SON-O) system of Holtzman rats in the first proestrus (PE), we demonstrated that Acetylcholine 10-6M (Ach) in CG increased in ovary the release of estradiol (E2) and nitric oxide (NO), a gaseous molecule with implications in steroidogenesis. The aim of this study was to study whether the described effect of Ach on E2 is per se or through the increase of NO. The system was incubated in Krebs-Ringer buffer at 37°C. Aminoguanidine 400 µM (AG) and L-nitroarginine methyl ester 100 µM (L-NAME), inhibitors of NO synthase, and sodium nitroprusside 100 µM (SNP), a exogenous NO donor, were added separately in O, with and without Ach 10-6M in CG. E2 release was determined by RIA at 30, 120 and 180 minutes of incubation, and P450 aromatase gene expression (P450arom, E2 synthesis enzyme) was determined by RT-PCR at 180 minutes. For statistical analysis of data ANOVA of repeated measures and Student´s t test (p