Acetylcholine in coeliac ganglion increases oxidative stress in ovarian: an effect modulated by nitric oxide

DELSOUC MB; DELLA VEDOVA C; RINALDI TOSI M; RAMÍREZ D; DELGADO SM

San Miguel de Tucumán

Congreso; 3ra Reunión Conjunta de Sociedades de Biología de la República Argentina; 2015

http://www.cricyt.edu.ar/biocell/39_5.pdf

Reactive oxygen species are necessary to lead to ovulation; however, with the reactive nitrogen species (RNS) they can cause cellular damage by reacting with macromolecules. Using the ex vivo coeliac ganglion-superior ovarian nerve-ovary (CG-SON-O) system of Holtzman rats in the first proestrus (PE), we demonstrated that stimulating the CG with acetylcholine (Ach) increases nitric oxide (NO) release, an RNS with implication in steroidogenesis and follicular development. The aim of this study was to evaluate if Ach 10-6M in CG modifies the oxidative state in O, in relation to changes in levels of NO. The system was incubated in Krebs-Ringer buffer at 37°C, 180 minutes. Aminoguanidine 400 µM (AG), selective inhibitor of inducible NO synthase, it was added both at O as in CG, separately, with and without addition of Ach 10-6 M in CG. Total antioxidant capacity (TAC), the activity of antioxidant enzymes catalase (CAT) and glutathione peroxidase (GSH-Px), and lipid peroxidation (TBARS) and protein oxidation (carbonyls) markers were determined in O homogenates. For statistical analysis of data ANOVA and Tukey test (p