Felis catus as a model to study follicle biology in vitro

ROJO, JL; LINARI, M; MUSSE, MP; PELUFFO, MC

JOURNAL OF ASSISTED REPRODUCTION AND GENETICS.

SPRINGER/PLENUM PUBLISHERS

Lugar: New York; Año: 2015 vol. 32 p. 1105 - 1111

1058-0468

AbstractPurpose The current study was designed to evaluate the responseof individual intact antral follicles from adult femaledomestic cats to a luteinizing hormone (LH) stimulus in vitroby assessing cumulus-oocyte expansion (C-OE) and steroidproduction.Methods C-OE and steroid levels (estradiol [E2] and progesterone[P4]) obtained from individual antral feline follicles(n=366 follicles; n=56 cats) were analyzed after 12 or 24 hof culture in the presence or absence of LH (low [3.4 ng/ml] orhigh [100 ng/ml]).Results At the end of the culture, the highest percentage ofexpanded cumulus-oocyte complexes (COCs) was observedin the LH groups at 12 or 24 h in comparison to their controls(p