INVESTIGADORES
GUGLIOTTA Agustina
artículos
Título:
Design and characterization of chimeric Rabies-SARS-CoV-2 virus-like particles for vaccine purposes
Autor/es:
GARAY, ERNESTO; FONTANA, DIEGO; VILLARRAZA, CARLOS JAVIER; FUSELLI, ANTONELA; GUGLIOTTA, AGUSTINA; ANTUÑA, SEBASTIÁN; TARDIVO, MARÍA BELÉN; RODRIGUEZ, MARÍA CELESTE; GASTALDI, VICTORIA; BATTAGLIOTTI, JUAN; ALVAREZ, DIEGO; CASTRO, ELIANA; CASSATARO, JULIANA; CEAGLIO, NATALIA; PRIETO, CLAUDIO
Revista:
Applied Microbiology and Biotechnology
Editorial:
Springer Science and Business Media Deutschland GmbH
Referencias:
Año: 2023 vol. 107 p. 3495 - 3508
Resumen:
great need for novel vaccine developments. In this field, there have been research approaches that focused on the productionof SARS-CoV-2 virus-like particles. These are promising vaccine candidates as their structure is similar to that of nativevirions but they lack the genome, constituting a biosafe alternative. In order to produce these structures using mammal cells,it has been established that all four structural proteins must be expressed. Here we report the generation and characterizationof a novel chimeric virus-like particle (VLP) that can be produced by the expression of a single novel fusion protein thatcontains SARS-CoV-2 spike (S) ectodomain fused to rabies glycoprotein membrane anchoring region in HEK293 cells. Thisprotein is structurally similar to native S and can autonomously bud forming enveloped VLPs that resemble native virionsboth in size and in morphology, displaying S ectodomain and receptor binding domain (RBD) on their surface. As a proofof concept, we analyzed the immunogenicity of this vaccine candidate in mice and confirmed the generation of anti-S, anti-RBD, and neutralizing antibodies.