Vascular tone is altered in animals with subtotal nephrectomy: the role of oxidative stress

LLICAS SANTIAGO; NEGRETE, ALEJANDRO; KARBINER M. SOFÍA; SALAS NICOLAS; MARAÑÓN, RODRIGO O.; CLAUDIO JOO TURONI; M. PERAL DE BRUNO

Tucuman

Jornada; XXIX Jornadas Científicas de la Asociación de Biología de Tucumán; 2012

Asociación de Biología de Tucumán

5/6 extraction of renal mass (nephrectomized rats: NefR) is an experimental model that develops chronic kidney disease (CKD). The development of hypertension (HT) and alteration of vascular function in NefR is controversial. It is known that vascular tone is altered in HT. Moreover, reduction of nitric oxide (NO) and oxidative stress alter vascular tone. Objectives: To study if NefR develop hypertension and to study the basal tone in aorta evaluating the role of NO and oxidative stress. Materials and Methods: In aortic rings from NefR we evaluated: 1) basal tone: response to sodium nitroprusside (SNP). 2) Vascular NO levels in basal conditions and in the presence of tempol or L-NAME. 3) Basal membrane potential (Pm). 4) glutathione/oxidized (GSH /GSSG) ratio and thiobarbituric acid reactive substances (TBARS) levels in aorta 5) NO and TBARS urinary levels. The results were compared to sham rats (SR). Results: NefR developed hypertension with decrease creatinine clearance. Aortic rings from NefR showed endothelial dysfunction, decreased NO levels (SR: 32±2 nA, n=7 vs. NefR: 10±2, n=8, P