Antioxidant treatment reverts increased arterial basal tone and oxidative stress in nephrectomized (5/6) hypertensive rats

MARAÑON RODRIGO; JOO TURONI CLAUDIO; KARBINER M. SOFÍA; SALAS NICOLAS; PERAL DE BRUNO MARIA

International Journal of Hypertension

Hindawi Publishing Corporation

Año: 2013 vol. 2013 p. 1 - 8

2090-0392

Non-ischemic 5/6 nephrectomized rat (NefR) is a model of chronic kidney disease.However, little is known about vascular dysfunction and its relation with hypertension in NefR. Aims: To evaluate possible alterations of endothelial function, NO-bioability and basal tone in aorta from NefR and the role of oxidative stress. Sprague Dawley rats were divided in: sham rats (SR), NefR and NefR treated with tempol (NefR-T). Mean arterial pressure (MAP) and renal function was determined.In isolated aortic rings was measured: 1-Endothelial function, 2-Basal tone, 3-NO levels, 4-Membrane potential (MP) and 5-Oxidative stress.NefR increased MAP (SR: 119±4 mmHg; n=7; NefR 169±6; n=8; p<0.001). Tempol did not modified MAP (NefR-T 168±10; n=6; p<0.001). NefR showed endothelialdysfunction, increased basal tone and decreased NO levels (SR: 32±2 nA; n=7,NefR: 10±2; n=8; p<0.001). In both in vitro and in vivo tempol improve basal tone,NO levels and MP. Oxidative stress in NefR was reverted in NefR-T. We described, for the first time, that aorta from NefR presented increased basaltone related to endothelial dysfunction and decreased NO-bioability. The fact thattempol improves NO-contents and basal tone, without decrease MAP, indicatesthat oxidative stress could be implicated early and independently to hypertension,in the vascular alterations.