Origen, adaptación y biogeografía del gen intI1

ÁLVAREZ VE; CHAMOSA LS; NARDELLI MAXIMILIANO; POWER P; QUIROGA MP; CENTRÓN D

Córdoba

Congreso; XI Congreso Argentino de Microbiología General SAMIGE; 2015

SAMIGE

Class1 integrons are themost successful mechanism associated to Lateral Antimicrobial Resistance GeneticTransfer among Gram-Negativeclinicalisolates. Recent studies showed they can befound in different habitats;however, a detailed study has not been conductedyet. The aim of this work wasto identify the origin and the variability ofcirculating alleles of intI1 type gene from class 1 integrons.A BLASTn query on GenBank using AF313471as reference was performed. AWKand Bioperlscripts, ClustalW, phylogenetic trees using Maximumlikelihood and bayesian methods and Jalview wereused in order to studycirculating alleles. Sixty eight alleles of intI1wereidentified.Althougha small number of alleles flowed in both habitats, they correspondedtothe 90.93% of the strains, suggesting the generalist intrinsic nature of thisgene, which is functional in different niches. These studies evidenced anactiveflow of intI1 alleles, both within the clinic and theenvironment andsuggest a scenario in which the first intI1allelesthat started tocirculate among clinical strains, have disseminated all around the world, withsporadic and continue captures of novel alleles from the open environment.