INVESTIGADORES
QUIROGA Maria Paula
congresos y reuniones científicas
Título:
Functional class 1 integrases of Pseudomonas spp. and Enterobacter spp. non-clinical isolates from non-urbanized sites of Patagonia: role as reservoir and source of antimicrobial resistance cassettes
Autor/es:
NARDELLI MAXIMILIANO; PREISEGGER MATÍAS; QUIROGA MARÍA PAULA; GALÁN ANGÉLICA VIVIANA; RAMÍREZ MARÍA SOLEDAD; CENTRÓN DANIELA
Lugar:
Primo?ten
Reunión:
Simposio; Central European Symposium On Antimicrobials And Antimicrobial Resistance (CESAR2012); 2012
Institución organizadora:
Croatian Microbiological Society in collaboration with Microbiological Societies from Czech Republic, Slovakia, Hungary and Bosnia and Herzegovina
Resumen:
(Presentación oral)   Functional class 1 integrases of Pseudomonas spp. and Enterobacter spp. non-clinical isolates from non-urbanized sites of Patagonia: role as reservoir and source of antimicrobial resistance cassettes.   Nardelli Maximiliano1, Preisegger Matías1, Quiroga María Paula1, Galán Angélica1, Ramírez María Soledad1, and Centrón Daniela1.   Instituto de Microbiología y Parasitología Médica, Universidad de Buenos Aires-Consejo Nacional de Investigaciones Científicas y Tecnológicas (IMPaM, UBA-CONICET), Facultad de Medicina, Ciudad Autónoma de Buenos Aires, Argentina.   Class 1 integrons are one of the most successful elements in the acquisition, expression and spread of antimicrobial resistance genes among gram-negative clinical isolates. Here, we demonstrated by Real-Time PCR, and PCR with the consequent sequence of the amplicons, that four intI1 genes from Pseudomonas spp., Enterobacter spp., Aranicola spp. and Agrobacterium tumefasciens environmental samples were able to insert in the attI1 site the aadB cassette that confers resistance to gentamicin. The frequency of recombination in the Pseudomonas spp. isolate was 10 to 100 times higher than in the other species. Although the plasmid carrying the aadB cassette could not be kept in the bacterial host, the four isolates maintained the aadB gene without antibiotic pressure up to 6 days. These results evidence the important role of class 1 integrons harboured by non-clinical isolates as reservoir and source for the flow of antimicrobial resistance gene cassettes among bacterial communities.