INVESTIGADORES
QUIROGA Maria Paula
congresos y reuniones científicas
Título:
Role of the secondary strucutre of the attC in RecA inpendent recombination events
Autor/es:
QUIROGA MARÍA PAULA; CENTRÓN DANIELA
Lugar:
Montevideo
Reunión:
Otro; advanced course working with pathogen genomes; 2008
Institución organizadora:
Wellcome Trust Fundation.
Resumen:
Integrons are mobilizable genetic structures associated with bacterial resistance to antibacterial agents, composed by the 5’ conserved sequence (5’-CS) including an integron integrase gene, a recombination site named attI and two divergent promoters, the 3’ conserved sequence (3’-CS) including genes which confer resistance to sulfonamides and antiseptics, and the variable region comprised between the 5´-CS and the 3´-CS, containing different rearrangements of gene cassettes. Gene cassettes confer resistance to almost all the families of antibiotics. Gene cassettes are promoterless mobile elements composed by an open reading frame and a recombination site named attC, which is recognized by the integron integrase to insert the gene cassette into the integron as well as to excise it from the integron. The attC sites vary from one another in their sequence and length generating diverse secondary structures and attC families. So far, those recombination sites are recognized by the integron integrases, by the SmaI2 group II intron and were recently found that they are also recognized by some insertion sequences like the IS1111-attC subfamily. I am currently studying which are the major features involved in the recognition of the integron recombination sites (attI and the different attC sites) by site specific recombinases. In my research work, I estimate the in vivo frequencies of the different recombination events and how they are affected when they are evaluated against the natural and the modified sequences and I look forward to evaluate how the diverse frequencies observed are related to the dispersion of the different elements.