Complex Class 1 Integrons with Diverse Variable Regions, Including aac(6')-Ib-cr, and a Novel Allele, qnrB10, Associated with ISCR1 in Clinical Enterobacterial Isolates from Argentina

MARÍA PAULA QUIROGA; PATRICIA ANDRÉS; ALEJANDRO PETRONI; ALFONSO J. C. SOLER BISTUÉ; LEONOR GUERRIERO; LILIANA JORDÁ VARGAS; ANGELES ZORREGUIETA; MARTA TOKUMOTO; CECILIA QUIROGA; MARCELO E. TOLMASKY; MARCELO GALAS; DANIELA CENTRÓN

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY

American Society for Microbiology

Año: 2007 vol. 51 p. 4466 - 4470

0066-4804

Transferable quinolone resistance has not previously been reported in Argentina. Here we describe three complex class 1 integrons harboring the novel allele qnrB10 in a unique region downstream of orf513, one of them also containing aac(6´)-Ib-cr within the variable region of integrons. The three arrays differed from blaCTX-M-2-bearing integrons, which are broadly distributed in Argentina. REFERENCES 1.      Arduino, S. M., P. H. Roy, G. A. Jacoby, B. E. Orman, S. A. Piñeiro, and D. Centrón. 2002. blaCTX-M-2 is located in an unusual class 1 integron (In35) which includes Orf513. Antimicrob. Agents Chemother. 46:2303-2306. 2.      Arduino, S. M., M. Catalano, B. E. Orman, P. H. Roy, and D. Centrón. 2003. Molecular epidemiology of orf513-bearing class 1 integrons in multiresistant clinical isolates from Argentinean hospitals. Antimicrob. Agents Chemother. 47:3945-3949. 3.      Cattoir V., L. Poirel, and P. Nordmann. 2007. Plasmid-Mediated quinolone resistance determinant qnrB4 in France from an Enterobacter cloacae clinical isolate co-expressing a qnrS1 determinant. Antimicrob. Agents Chemother. 51:2652-2653. 4.      Clinical and Laboratory Standards Institute (CLSI). 2007. Performance standards for antimicrobial susceptibility testing; seventeenth informational supplement M100-S17. vol. 27, no. 1. CLSI. Wayne (PA). 5.      Garnier, F., N. Raked, A. Gassama, F. Denis, and M. C. Ploy. 2006. Genetic environment of quinolone resistance gene qnrB2 in a complex sul1-type integron in the newly described Salmonella enterica serovar Keurmassar. Antimicrob. Agents Chemother. 50:3200-3202. 6.      Gay, K., A. Robicsek, J. Strahilevitz, C. H. Park, G. Jacoby, T. J. Barrett, F. Medalla, T. M. Chiller, and D. C. Hooper. 2006. Plasmid-mediated quinolone resistance in non-typhi serotypes of Salmonella enterica. Clin. Infect. Dis. 43:297–304. 7.      Jacoby, G. A., K. E. Walsh, D. M. Mills, V. J. Walker, H. Oh, A. Robicsek, and D. C. Hooper. 2006. qnrB, another plasmid-mediated gene for quinolone resistance. Antimicrob. Agents Chemother. 50:1178-1182. 8.      Levesque, C., L. Piche, C. Larose, and P. H. Roy. 1995. PCR mapping of integrons reveals several novel combinations of resistance genes. Antimicrob. Agents Chemother. 39:185-191. 9.      Martinez-Freijo, P., A. C. Fluit, F. J. Schmitz, J. Verhoef, and M. E. Jones. 1999. Many class I integrons comprise distinct stable structures occurring in different species of Enterobacteriaceae isolated from widespread geographic regions in Europe. Antimicrob. Agents Chemother. 43:686-689. 10.  Melano, R., A. Corso, A. Petroni, D. Centrón, B. Orman, A. Pereyra, N. Moreno, and M. Galas. 2003. Multiple antibiotic-resistance mechanisms including a novel combination of extended-spectrum β-lactamases in a Klebsiella pneumoniae clinical strain isolated in Argentina. J. Antimicrob. Chemother. 52:36–42. 11.  Orman, B. E., S. A. Piñeiro, S. Arduino, M. Galas, R. Melano, M. I. Caffer, D. O. Sordelli, and D. Centrón. 2002. Evolution of multiresistance in nontyphoid Salmonella serovars from 1984 to 1998 in Argentina. Antimicrob. Agents Chemother. 46:3963-3970. 12.  Périchon, B., P. Courvalin, and M. Galimand. 2007. Transferable resistance to aminoglycosides by methylation of G1405 in 16S rRNA and to hydrophilic fluoroquinolones by QepA mediated efflux in Escherichia coli. Antimicrob. Agents. Chemother. 51:2464-2469. 13.  Poirel, L., A. Liard, J. M. Rodriguez-Martinez, and P. Nordmann. 2005. Vibrionaceae as a possible source of Qnr-like quinolone resistance determinants. J. Antimicrob. Chemother. 56:1118-1121. 14.  Robicsek, A., J. Strahilevitz, G.A. Jacoby, M. Macielag, D. Abbanat, C. H. Park, K. Bush, and D. C. Hooper. 2006. Fluoroquinolone-modifying enzyme: a new adaptation of a common aminoglycoside acetyltransferase. Nat. Med. 12:19-20. 15.  Robicsek, A., G. A. Jacoby, and D. C. Hooper. 2006. The worldwide emergence of plasmid-mediated quinolone resistance. Lancet Infect. Dis. 6:629–640. 16.  Toleman, M. A., P. M. Bennett, and T. R. Walsh. 2006. ISCR elements: novel gene-capturing systems of the 21st century? Microbiol. Mol. Biol. Rev. 70:296-316.17.  Verdet, C., Y. Benzerara, V. Gautier, O. Adam, Z. Ould-Hocine, and G. Arlet. 2006. Emergence of DHA-1-producing Klebsiella spp. in the Parisian region: genetic organization of the ampC and ampR genes originating from Morganella morganii. Antimicrob. Agents Chemother. 50:607-617. 18.  Wang, M., J. H. Tran, G. A. Jacoby, Y. Zhang, F. Wang, and D. C. Hooper. 2003. Plasmid-mediated quinolone resistance in clinical isolates of Escherichia coli from Shanghai, China. Antimicrob. Agents Chemother. 47:2242-2248. 19.  Yamane, K., J. Wachino, S. Suzuki, K. Kimura, N. Shibata, H. Kato, K. Shibayama, Konda T, and Y. Arakawa. 2007. New Plasmid-Mediated Fluoroquinolone Efflux Pump, QepA, Found in an Escherichia coli Clinical Isolate. Antimicrob. Agents. Chemother. 51:3354-3360.