THE PROTEIN AsES ACTIVATES THE INNATE IMMUNITY IN STRAWBERRY

DÍAZ RICCI, JUAN C.; PERATO, SILVIA M.; HAEL CONRAD, VERÓNICA; MARTOS, GUSTAVO G.

Bueno Aires

Jornada; XX JORNADAS ANUALES DE LA SOCIEDAD ARGENTINA DE BIOLOGÍA (SAB); 2018

SOCIEDAD ARGENTINA DE BIOLOGÍA (SAB)

We have previously reported that strawberry plants (Fragaria ananassa) treated with the defense elicitor AsES exhibited a higherresistance against the fungal pathogens Colletotrichum actutatum and Botrytis cinerea. It was hypothesized that the effect was due to theactivation of the plant innate immunity. With the aim to test that hypothesis we have investigated which defense signaling pathway wasactivated in plants treated with AsES. Strawberry leaves of the cv Pájaro were treated w ith AsES and harvested at different times toevaluate the level of expression of different genes associated to the salicylic acid (SA) and ethylene (ET) signaling pathways. Plantstreated with water were used as control. The evaluated genes associated to the SA pathway were: PR1, ISC1, PAL1, RBOHD-F, CHI1.2,SAMT, SAMES and SAGT; and the genes associated to the ET pathway were: ETR1, ACS, ACO, CTR1, ERS1, EIN2, PDF1.2. Othergenes associated to defense response such as: YB30 (HR marker); FLS (flavonol synthesis); GSL5 (callose synthesis); PRX27 (ligninsynthesis), and PG (cell wall degradation) were also investigated. The accumulation of SA and ET production were also analyzed inplants at different times after AsES treatment. Results obtained revealed that AsES activates the innate immunity by using, in an earlystage, a mechanism independent to RBOHD and RBOHF, that is an oxidative burst independent to NADPH oxidase, and activates bothsignaling pathways (i.e. SA and ET), but in a sequential mode, revealing a cross-talk between them.