INVESTIGADORES
DIAZ RICCI Juan Carlos
congresos y reuniones científicas
Título:
Antioxidant activity and SA accumulation associated to the defense response in strawberry
Autor/es:
TONELLO, URUSULA; MAMANI, MA. ALICIA; SALAZAR, SERGIO MIGUEL; GRELLET, CARLOS; TORTORA, MA. LAURA; CASTAGNARO, ATILIO PEDRO; DIAZ RICCI, JUAN CARLOS
Lugar:
Rosario, Argentina
Reunión:
Congreso; 42 Reunión Anual de la Sociedad Argentina de Investigaciones en Bioquímica y Biología Molecular (SAIB); 2006
Institución organizadora:
Sociedad Argentina de Investigaciones Bioquímicas
Resumen:
Plant resistance to hemibiotrophic fungi is a poorly understood process. In a previous study, we found that the isolate M23 of Colletotrichum presents an incompatible interaction with Fragaria ananassa cv Pájaro. To isolate genes differentially induced in the incompatible interaction we have used mRNA differential display. This technique was performed with total RNA prepared from strawberry leaves 48 hai. The cDNAs of interest were excised from the gel, reamplified, cloned and sequenced. One clone named I (6,5)2B carrying an insert of 288 bp showed 50% identity with GST 18 from Glycine max and other plant species. An increase of GSTs expression after pathogen attack was reported in several plant-pathogen interactions. It has also been postulated that antioxidant activity of GSTs plays an important role in limiting the damage caused by ROS. Positive DAB and NBT staining were observed in strawberry leaves 2-4 hai with M23. Leaves extracts were assessed for TBA/MDA reaction showing peaks at 4 and 24 ha and a decrease 48 hai, accompanied by ascorbate peroxidase activity. Results also revealed a peak of free and conjugated salicylic acid (SA) at 48 hai in phloem exudates. Taken together these results suggest the involvement of complex redox regulation in the establishment of the defense response in strawberry.