INVESTIGADORES
DIAZ RICCI Juan Carlos
congresos y reuniones científicas
Título:
ROS ACTIVATED IN STRAWBERRY PLANTS BY SMS IS SUPPRESSED BY SUPERNATANT OF COLLETOTRICHUM ACUTATUM
Autor/es:
TOMAS GRAU, RODRIGO; REQUENA SERRA, FERNANDO J.; GUERRERO MOLINA, MA. FERNANDA; PERATO, MARISA; MARTINEZ ZAMORA, GUSTAVO; DÍAZ RICCI, JUAN C.
Lugar:
Rosario, Santa Fe.
Reunión:
Congreso; SAIB 51º Annual Meeting; 2014
Institución organizadora:
Argentine Society for Biochemistry and Molecular Biology
Resumen:
The generation of Reactive Oxygen Species (ROS) is one of the early defense responses activated in plants by biotic and abiotic stresses. Previously, we observed ROS production and up-regulation of catalase and pathogenesis related protein 1 (FaCAT and FaPR1) genes in strawberry leaves subjected to Soft Mechanical Stimulation (SMS, softly pressing the leaves with the fingers). The aim of this work was to evaluate the effect of treatment with the supernatant of the virulent isolate M11 of Colletotrichum acutatum (SN-M11) on the biochemical and molecular responses of SMS in strawberry. Four treatments were applied to axenic in vitro strawberry plants by triplicate: 1) SN-M11, 2) SMS; 3) SN-M11 and 4 hours later SMS and 4) H2O. Leaves where then removed for histochemical staining (NBT and DAB) and RNA extraction. The expression of FaCAT and FaPR1 genes were analyzed by qPCR. Results showed that leaves treated with the SN-M11 exhibited lesser H2O2 and O2- production than SMS treated plants, showing also a down-regulation of FaCAT transcripts. Nevertheless, up-regulation of FaPR1 was observed in treatments (2) and (3) as compared to (1) and (4). These results suggest that SN-M11 suppresses the production of ROS but not the defensive response activated by SMS