Evaluation of oxidative stress in strawberry plant cell suspensions

MARTOS, GUSTAVO G.; DÍAZ RICCI, JUAN C.

Tafí del Valle, Tucumán

Jornada; 28º Annual Scientific Meeting; 2011

Tucumán Biology Associatioin

Plants under biotic or abiotic stress react by producing a series ofbiochemical reactions that can lead to a defense response and themeasurement of reactive oxygen species (ROS) such as H2O2 isvery useful to study this plant response. In this work we present asystem to study the intracellular level of H2O2 in plant cell suspensions.Mesophyll cells of 8-week-old strawberry plants (Fragaria xananassa) of the cv Pájaro were used. 1.5g of leaves were washed,cut into squares, and homogenized in a potter with 10 mL of bufferW5. Cells were recovered by differential centrifugation. The levelof H2O2 was measured by fluorometry and cellular viability wasdetermined with Blue Evans dye. The suspension (1.5 x 106 cell/mL) was evaluated for 24 hours. Results showed that the initiallevel of H2O2 decreased approximately 65% after a night in thedark at 25°C and cell viability decreased 8% 24 h after extraction.The system was induced with Paraquat (0.1 mM) showing a peakof H202 production 3 hours after the induction and the effect wascompletely suppressed when ascorbic acid (5 mM) was added 30min previous to Paraquat. These results let us conclude that thesystem of cell suspension developed constitutes a useful and reliablemethod to study stress in plants.