INVESTIGADORES
DIAZ RICCI Juan Carlos
congresos y reuniones científicas
Título:
IN VITRO REGENERATION OF FRAGARIA VESCA PLANTS FROM LEAF AND PETIOLE EXPLANTS
Autor/es:
HAEL CONRAD, VERÓNICA; TOMAS GRAU, RODRIGO; DÍAZ RICCI, JUAN C.
Lugar:
Tafí del Valle. Tucumán.
Reunión:
Jornada; 29º Annual Scientific Meeting; 2012
Institución organizadora:
Tucumán Biology Associatioin
Resumen:
An efficient regeneration protocol is essential for the successful genetic transformation of plants. The objective of this work was to adjust a plant regeneration system from leaf and petiole of local accessions of F. vesca. Fruits were collected from the location of Villa Nougués, achenes were extracted, scarified with sulfuric acid (96%) and plated on two media cultures: half-strength MS 4% sucrose (MS1), and MS 3% sucrose (MS2) (both pH 5.74). Cultures were maintained at 25°C and a photoperiod of 16h light/day (40 μmoles/m2.s). After 4 months MS1 was chosen because plants grown in this medium were larger. Leaf and petiole sections were excised from 4-months-old seedlings and cultured on regeneration medium (MS with 3% sucrose, 3mg/I BAP and 0.25mg/l IBA for leaves, and 1mg/I BAP and 0.25mg/l IBA for petioles, pH 5.74). Leaves and petioles regenerated as masses of undifferentiated cells called callus. 70% of leaves callus and 50% of petioles callus gave shoots. 5mm-height-shoots were excised and cultured in MS1. Rooted plants were rusticated into sterile peat, kept in humid chamber under the conditions mentioned above, and one month later placed in greenhouse. Results obtained let us to select a suitable culture medium for in vitro germination of achenes of F. vesca, and to adjust an efficient plant regeneration system from leaf and petiole explants.