INVESTIGADORES
DIAZ RICCI Juan Carlos
congresos y reuniones científicas
Título:
SYNTHESIS OF QUORUM SENSING SIGNALS BY
Autor/es:
NIETO PEÑALVER, CARLOS G.; BICHARA, L.; MARINO, D.; CASTELLANOS DE FIGUEROA, LUCÍA; DÍAZ RICCI, JUAN C.
Lugar:
Puerto Madryn
Reunión:
Congreso; 46º Reunión Anual de la Sociedad Argentina de Investigaciones en Bioquímica y Biología Molecular (SAIB); 2010
Institución organizadora:
Sociedad Argentina de Investigaciones en Bioquímica y Biología Molecular (SAIB)
Resumen:
In the rhizosphere, interactions occur with both pathogenic and
beneficial microorganisms. The latter group can stimulate plant
growth by mean of phytohormone production, direct antagonism of
pathogens or induction of systemic resistance in the plant. Quorum
sensing (QS) systems from non symbiotic diazotrophic bacteria may
play a role in the interactions with strawberry plants and with other
organisms. The objective of this work was the characterization of Nacylhomoserine
lactones (N-AHLs) produced by free living
nitrogen fixing bacteria associated to roots of Fragaria ananassa
Duch. cv. Camarosa. Strains were isolated and purified in nitrogen
free semisolid media. Extracts were prepared with acidified ethyl
acetate and samples were analyzed by TLC. Chromobacterium
violaceum Vir07, Agrobacterium tumefaciens NTL4 (pCF218
pCF372) and Pseudomonas putida F117 (pKR-C12) were utilized
as biosensor strains for the detection of QS molecules. Nonsymbiotic
diazotrophic bacteria producing QS molecules could be
isolated from roots of strawberry plants. Production of short chain
N-AHLs was detected in all strains. Only one strain produced long
chain N-AHLs. Although biosynthesis of QS molecules is not
widely distributed in diazotrophic strains from strawberry roots,
secretion of short chain QS molecules seem to play a key role as
signaling molecules.