Effect of brassinosteroids treatment in strawberry defense markers

FURIO, RAMIRO N.; YAMILET COLL; MARTINEZ ZAMORA, GUSTAVO; DÍAZ RICCI, JUAN C.; SALAZAR, SERGIO M.

Córdoba

Congreso; 52º Reunión Anual de la Sociedad Argentina de Investigaciones en Bioquímica y Biología Molecular (SAIB).; 2016

SAIB

Brassinosteroids (BRs) are steroidal compounds that are essential for plant growth and development. It was shown thatBRs can also provide plants tolerance/resistance to a wide range of biotic and abiotic stresses in a dose dependent manner. The aim of this work was to evaluate some defence biochemical markers such as H2O2, O2-andcallose in strawberry plants of the cv. Pájaro treated with the BRs 24-epibrasinolide (EP24) and the commercial formulation BIOBRAS 16 (BB16). Defence related oxidative burst was evaluated by two histochemical staining, and H2DCF-DAfluorescent probe. Callose deposition was studied by histochemical staining with aniline blue. The influence of BRs on stomatal aperture was evaluated in epidermal strips of Vicia faba. Results show that strawberry plants treated with EP24 and BB16 showed increased production of H2O2, and O2-, and higher callose deposition as compared to the control plants. However, stomatal closure was only observed in leaf strips treated with BB16. These results reveal that BRs can also be used for the activation of innate immunity in strawberry plants, hence could be used as a new strategy for the control of strawberry plants diseases, environmentally friendly and alternative to agrochemicals.