INVESTIGADORES
DIAZ RICCI Juan Carlos
artículos
Título:
Design of stable metabolic networks
Autor/es:
MAGGIO, JIMENA DI; BLANCO, ANÍBAL M.; BANDONI, J. ALBERTO; DÍAZ RICCI, JUAN CARLOS; DIAZ, M. SOLEDAD
Revista:
ENGINEERING IN LIFE SCIENCES
Editorial:
WILEY-V C H VERLAG GMBH
Referencias:
Lugar: Weinheim; Año: 2017 vol. 17 p. 908 - 915
ISSN:
1618-0240
Resumen:
In this work, we propose eigenvalue optimization combined with Lyapunov theory conceptstoensurestabilityoftheEmbden?Meyerhof?Parnaspathway,thepentosephosphate pathway, the phosphotransferase system and fermentation reactions of Escherichia coli. We address the design of a metabolic network for the maximization of different metabolite production rates. The first case study focuses on serine production,basedonamodelthatconsistsof18differentialequationscorresponding todynamicmassbalancesforextracellularglucoseandintracellularmetabolites,and thirty kinetic rate expressions. A second case study addresses the design problem to maximize ethanol production, based on a dynamic model that involves mass balancesfor25metabolitesand38kineticrateequations.Thenonlinearoptimization problemincludingstabilityconstraintshasbeensolvedwithreducedspaceSuccessive Quadratic Programming techniques. Numerical results provide useful insights on the stability properties of the studied kinetic models.