INVESTIGADORES
DIAZ RICCI Juan Carlos
artículos
Título:
Demonstration of the Presence of an Inducible B-3-Lactamase in Azospirillum lipoferum
Autor/es:
BOGGIO SILVANA B.; DÍAZ RICCI, JUAN C.; DE MENDOZA, DIEGO; ROVERI, OSCAR A.
Revista:
CURRENT MICROBIOLOGY
Editorial:
SPRINGER
Referencias:
Lugar: Berlin; Año: 1989 vol. 18 p. 33 - 35
ISSN:
0343-8651
Resumen:
Abstract. Azospirillum lipoferum is a soil microorganism that has been shown to beresistant topenicillins. It has been suggested thatresistance is due to the presence of a B-1actamase activity,but no conclusive evidence has beenreported. The incubation of benzylpenicillin or nitrocephinwith either whole Azospirillum cells or cell-free extracts was accompanied byhydrolysis of theB-1actam ring of the antibiotics. Suchhydrolytic activity exhibited Michaelis and Menten-likekinetics. The enzyme was produced at a low,basal level that was increased approximately 50times by the addition of benzylpenicillin,an increase that was completely blocked by chloramphenicolor rifampicin.