INVESTIGADORES
DIAZ RICCI Juan Carlos
artículos
Título:
Metabolic enhancement due to plasmid maintenance
Autor/es:
RHEE, J.L.; DÍAZ RICCI, JUAN C.; BODE, J.; SCHÜGERL, K
Revista:
BIOTECHNOLOGY LETTERS
Editorial:
SPRINGER
Referencias:
Lugar: Berlin; Año: 1994 vol. 16 p. 881 - 884
ISSN:
0141-5492
Resumen:
Plasmid maintenance allows the strain JM109 of Escherichia coli to grow in a minimal defined medium (M9). JM109 carrying no plasmid can hardly grow in M9 whereas JM109 carrying one, two and three plasmids have a clear metabolic over the untransformed strain. In a complex medium like LB (Luria-Bertani Broth) all strains grow well and despite the number of plasmids carried by the host maximum specific growth rates are not severely affected. Our results suggest that the glucose metabolism is an essential factor contributing to this behavior.
