INVESTIGADORES
DIAZ RICCI Juan Carlos
artículos
Título:
Physiological, structural and molecular traits activated in strawberry plants after inoculation with the plant growth promoting bacterium Azospirillum brasilense REC3
Autor/es:
GUERRERO MOLINA, MA. FERNANDA; LOVAISA, NADIA; SALAZAR, SERGIO M; MARTÍNEZ ZAMORA, MARTÍN G.; DÍAZ RICCI, JUAN C.; PEDRAZA, RAÚL O.
Revista:
PLANT BIOLOGY
Editorial:
WILEY-BLACKWELL PUBLISHING, INC
Referencias:
Lugar: Londres; Año: 2015 vol. 17 p. 766 - 773
ISSN:
1435-8603
Resumen:
The plant growth-promoting strain REC3 of Azospirillum brasilense, isolated fromstrawberry roots, prompts growth promotion and systemic protection againstanthracnose disease in this crop. Hence, we hypothesised that A. brasilense REC3 caninduce different physiological, structural and molecular responses in strawberryplants. Therefore, the aim of this work was to study these traits activated in Azospirillum-colonised strawberry plants, which have not been assessed until now. Healthy,in vitro micropropagated plants were root-inoculated with REC3 under hydroponicconditions; root and leaf tissues were sampled at different times, and oxidative burst,phenolic compound content, malondialdehyde (MDA) concentration, callose deposition,cell wall fortification and gene expression were evaluated. Azospirillum inoculationenhanced levels of soluble phenolic compounds after 12 h post-inoculation (hpi),while amounts of cell wall bound phenolics were similar in inoculated and controlplants. Other early responses activated by REC3 (at 24 hpi) were a decline of lipid peroxidationand up-regulation of strawberry genes involved in defence (FaPR1), bacterialrecognition (FaFLS2) and H2O2 depuration (FaCAT and FaAPXc). The last mayexplain the apparent absence of oxidative burst in leaves after bacterial inoculation.Also, REC3 inoculation induced delayed structural responses such as callose depositionand cell wall fortification (at 72 hpi). Results showed that A. brasilense REC3 iscapable of exerting beneficial effects on strawberry plants, reinforcing their physiologicaland cellular characteristics, which in turns contribute to improve plantperformance.
