INVESTIGADORES
DIAZ RICCI Juan Carlos
artículos
Título:
Magneto-mechanical model of an enzyme.
Autor/es:
JUAN C. DIAZ RICCI
Revista:
Chemical Educator
Editorial:
SPRINGER-VERLAG
Referencias:
Lugar: New York; Año: 2002 vol. 7 p. 7 - 10
ISSN:
1430-4171
Resumen:
A genetically structured mathematical model is developed and used to evaluate the influence of molecular parameters involved in the expression of a harmful recombinant protein (SPA::EcoRI). The system consists of the controlled expression of the endonuclease EcoRI cloned in the plasmid pMTC48. The control is exerted by the lambda CI repressor expressed from the plasmid pRK248cIts. The deleterious effect of the activity of the enzyme EcoRI on the host DNA is prevented by the action of the EcoRI methylase that is expressed constitutively from a third plasmid, pEcoR4. The model includes molecular mechanisms involved in the regulation of the expression of these genes and is used to determine cultural conditions that maximize the production of the recombinant protein.
