Modeling of the Controlled Expression of a Harmful Protein by a Three Plasmid Harboring System.

UNZAGA, T.V.; DIAZ RICCI, JUAN C; RHEE, J.L.; HERNÁNDEZ, M.R.; SCHÜGERL, K

BIOENGINEERING AND BIOTECHNOLOGY

JOHN WILEY & SONS INC

Lugar: Philadelphia; Año: 2002 vol. 80 p. 544 - 551

0006-3592

A genetically structured mathematical model is developed and used to evaluate the influence of molecular parameters involved in the expression of a harmful recombinant protein (SPA::EcoRI). The system consists of the controlled expression of the endonuclease EcoRI cloned in the plasmid pMTC48. The control is exerted by the lambda CI repressor expressed from the plasmid pRK248cIts. The deleterious effect of the activity of the enzyme EcoRI on the host DNA is prevented by the action of the EcoRI methylase that is expressed constitutively from a third plasmid, pEcoR4. The model includes molecular mechanisms involved in the regulation of the expression of these genes and is used to determine cultural conditions that maximize the production of the recombinant protein.