INVESTIGADORES
DIAZ RICCI Juan Carlos
artículos
Título:
Effect of Fragarin on the cytoplasmic membrane of the phytopahogen Clavibacter michiganensis.
Autor/es:
FILIPPONE, M.P.; DÍAZ RICCI, J.C:; CASTAGNARO, A.; FARÍAS, R.; JUAN C. DIAZ RICCI
Revista:
MOLECULAR PLANT-MICROBE INTERACTIONS
Editorial:
AMER PHYTOPATHOLOGICAL SOC
Referencias:
Lugar: Washington; Año: 2001 vol. 14 p. 925 - 928
ISSN:
0894-0282
Resumen:
Fragarin, an antibiotic that was isolated and purified from a soluble fraction of strawberry leaves, may be a new type of preformed antimicrobial compound (phytoanticipin). Here, we report that the growth and oxygen consumption of the phytopathogenic bacterium Clavibacter michiganensis were rapidly inhibited after the addition of fragarin to cultures. Also, dissipation of the membrane potential and an increase of cell membrane permeability were observed in the presence of fragarin. The ability of fragarin to dissipate the membrane potential was confirmed with the use of small unilamellar liposomes made with lipids extracted from C. michiganensis. Our results suggest that fragarin is able to act at the membrane level, and that this action is correlated with a decrease in cell viability.
