INVESTIGADORES
DIAZ RICCI Juan Carlos
artículos
Título:
White-Fruited Duchesnea Indica (Rosaceae) impaired in the ANS Gene Expression
Autor/es:
DEBES, MARIO A.; ARIAS, MARTA E; GRELLET BOURNONVILLE, CARLOS F.; WULFF, A.F. ; MARTÍNEZ ZAMORA, MARTÍN G.; CASTAGNARO, ATILIO P; DÍAZ RICCI, JUAN C.
Revista:
AMERICAN JOURNAL OF BOTANY
Editorial:
BOTANICAL SOC AMER INC
Referencias:
Lugar: New York; Año: 2011 vol. 98 p. 2077 - 2083
ISSN:
0002-9122
Resumen:
Premise of the study: Duchesnea indica is a wild strawberry-like species that exhibits red-fruits. In a survey we have detected a white-fruited individual. The aim of this study was to investigate whether the white-fruited character was due to a phenotypical or genotypical change. The stability, heritability and genes involved in the anthocyanins synthesis was studied in comparison with red-fruited genotypes. This study contributes to understand the molecular basis of some factors involved in the fruit pigmentation and the heritability of the white-fruited character. Methods: The stability and heritability of the white-fruited character were evaluated in plants obtained by asexual propagation or by sexual crosses between the white- and red-fruited genotypes. Asexual multiplication were carried by stolon rooting and sexual by germination of achenes obtained from crosses. The expression level of the genes involved in the synthesis and regulation of anthocyanins pathway (CHS, F3H, DFR, ANS and MYB10) were evaluated by RT-PCR using specific primers. Key results: Plants exhibiting the white-fruited character always yielded white-fruited progeny when propagated asexually, whereas in sexually propagated plants depended on the mother used. Red-fruited mother yielded red-fruited progeny and white-fruited mother yielded fruits from dark pink to white color. Molecular analysis suggested that the white-fruited character was due to the low expression of the ANS gene. Conclusions: Results obtained indicate that the white-fruited character was stable. Mother progenitors exert a strong influence on the expression of the white-fruited character. The white-fruited phenotype is due to the impairment or down regulated of the ANS gene.